cancerx.org TumorX salve and a cancer protocol including enzymes, minerals, and a treatment for cachexia, a CD for treatment advice, books and other materials.

Bloodroot cancer salve treatment has been "orthodox" medicine in the Philippines since 2002

TumorX Products are Avaliable World Wide
Jade Del Mundo Md. From the Department of Health invites you to the Philippines for your healing.

Philippine Department of Health

Excising news from the Philippine College For The Advancement In Medicine Foundation Inc. During their fourth annual meeting November 13-16, 2005 held at the Subic International Hotel, the Philippine Undersecretary of Health of the Office for Special Concerns, Dr. Jade F. Del Mundo M.D., F.P.B.O., F.P.C.S. ... announced that natural alternative cancer treatments like the TumorX Protocol are welcome to be practiced by qualified practitioners in the Philippines.

TumorX Paste has been used in the Philippines since 2002. The first patient who used the TumorX Paste was a lady with an ulcerated breast cancer, she had already used

radiation and chemotherapy with no positive results at a local Manila hospital. Dr. Merced decided after reviewing her case, and consulting with peers in the Philippines and around the world, that her patient’s only hope of survival ... was to use an alternative protocol. Dr. Merced successfully use the TumorX Paste Protocol. After numerous applications of the paste the treatment was a complete success!

She, the patient is teaching at a prominent college in Manila thanks to the dedicated work of her doctor Fe Jocelyn G. Merced, M.D.

To see contact information and Biographer data on a potential Philippine Doctor click on their name. (go to the cancerx.org site for the actual links)

Dr. Carmencita R. Yap, M.D.
Dr. Merced, M.D.
Dr. Lemuel Tocjayao, M.D.

Growing and Marketing Woodland Botanicals Jeanine M. Davis
http://www.hort.purdue.edu/newcrop/ncnu07/pdfs/davis274-279.pdf
Bloodroot (Sanguinaria canadensis L., Papaveraceae) is one of the first plants to emerge and bloom in the spring. It is a low growing plant with waxy lobed leaves and showy white flowers. The thick rhizome is dark on the outside, with a cream colored flesh and blood red sap (Fig. 4). The rhizome has traditionally been used as a dye, to treat skin lesions, and to prevent tooth decay (Persons and Davis 2005). The demand for bloodroot has been low but steady for many years, and as a result, it has been almost exclusively wild harvested. This changed dramatically when a German company began using it as an appetite stimulant in cattle feed. It is now also being studied for the treatment of cancer (e.g., Ahmad et al. 2000). The major alkaloid believed to be responsible for its medicinal and anti-microbial properties is sanquinarine (Leung and Foster 1996). Bloodroot studies are in progress on seed and vegetative propagation, tillage systems, and companion planting with other woodland botanicals (Persons and Davis 2005). Bloodroot is commonly propagated by rhizome cuttings and seed.

American Association For Cancer Research
http://clincancerres.aacrjournals.org/cgi/content/full/6/4/1524#ABS
Nihal Ahmad, Sanjay Gupta, Mirza M. Husain, Kaisa M. Heiskanen and Hasan Mukhtar2
Department of Dermatology [N. A., S. G., M. M. H., H. M.] and Department of Anatomy, School of Medicine [K. M. H.], Case Western Reserve University, Cleveland, Ohio 44106

ABSTRACT
Sanguinarine, derived from the root of Sanguinaria canadendid, has been shown to possess antimicrobial, anti-inflammatory, and antioxidant properties. Here we compared the antiproliferative and apoptotic potential of sanguinarine against human epidermoid carcinoma (A431) cells and normal human epidermal keratinocytes (NHEKs). Sanguinarine treatment was found to result in a dose-dependent decrease in the viability of A431 cells as well as NHEKs albeit at different levels because sanguinarine-mediated loss of viability occurred at lower doses and was much more pronounced in the A431 carcinoma cells than in the normal keratinocytes. DNA ladder assay demonstrated that compared to vehicle-treated control, sanguinarine treatment of A431 cells resulted in an induction of apoptosis at 1-, 2-, and 5-µM doses. Sanguinarine treatment did not result in the formation of a DNA ladder in NHEKs, even at the very high dose of 10 µM. The induction of apoptosis by sanguinarine was also evident by confocal microscopy after labeling the cells with annexin V. This method also identified necrotic cells, and sanguinarine treatment also resulted in the necrosis of A431 cells. The NHEKs showed exclusively necrotic staining at high doses (2 and 5 µM). We also explored the possibility of cell cycle perturbation by sanguinarine in A431 cells. The DNA cell cycle analysis revealed that sanguinarine treatment did not significantly affect the distribution of cells among the different phases of the cell cycle in A431 cells. We suggest that sanguinarine could be developed as an anticancer drug.

http://clincancerres.aacrjournals.org
3176 Vol. 9, 3176–3182, August 1, 2003 Clinical Cancer Research
Vaqar Mustafa Adhami, Moammir Hasan Aziz,
Hasan Mukhtar, and Nihal Ahmad2
Department of Dermatology, University of Wisconsin, Madison,
Wisconsin 53706
Activation of Prodeath Bcl-2 Family Proteins and Mitochondrial Apoptosis Pathway by Sanguinarine in iimmortalized Human HaCaT Keratinocytes1 (click link to go to article site)

http://www.cancerx.org/science_of_bloodroot.html
A summary of research findings
From escharotic salve expert Dan Raber's website:

"INTRODUCTION TO BLOODROOT"
Sanguinarine canadendid (bloodroot) is native to the moist woodlands of Eastern U.S. and Canada. This small North American herb is a member of the Papaveraceae Herbaceous perennial (poppy family), and possess attributes that are remarkable and almost unbelievable. Numerous doctors and researchers have investigated Sanguinarine canadendid, i.e., 13-methyl[1,3]benzodioxolo[5,6-c]-1,3-dioxolo[4,5-i]phenanthridinium; the sap of the plant is bright red and is especially abundant with the alkaloid sanguinarine which is derived from rhizome (root system ), a benzophenanthridine alkaloid and a structural homologue of chelerythrine. Sanguinarine is known for its anti-cancer/anti-tumor, anti-inflammatory, anti-microbial (Sanguinarine broad in vitro activity against Gram-positive and Gram-negative bacteria, fungi, and some protozoa), anti-oxidant, and expectorant properties.

The effect of sanguinarine and its antiproliferative and apoptosis nature was investigated and the following found to be true:

1. Sanguinarine stops cancerous cells rapid growth.
2. Sanguinarine stops cancerous cells from abnormally increasing in number.
3. Sanguinarine stops the promotion of human epidermoid carcinoma cells, i.e., squamous cell (squamous cell is an invasive malignant tumor derived from epithelial tissue that tends to metastasize to other areas of the body).
4. Sanguinarine promotes the natural self-destruction (apoptosis) of cancer cells.
5. Sanguinarine will not promote apoptosis at any strength or concentration to health tissue.
6. Sanguinarine will promote necrosis (cell death ) in healthy epidermal keratinocytes (keratinocytes are the predominant cell type in the epidermis and dermis i.e. skin tissue) in concentrations above -10 µM (10-6 per unit).

Safety of Sanguinarine
The safety of sanguinarine has been demonstrated with over 400 years of use by professionals and novices alike. Sanguinaria extract, sanguinarine, has been used in many over-the-counter products, including toothpaste (the anti-inflammatory properties in human's to reduce gingival inflammation and supragingival plaque.), mouthwash, cough and cold remedies, and homeopathic preparations for cancer removal.

Sanguinaria Kills Squamous Cell Carcinoma
Sanguinarine treatment was found to result in a dose-dependent decrease in the viability of squamous cell carcinoma. Normally cancerous cells are unable to experience apoptosis by natural means. Sanguinarine (13-methyl[1,3]benzodioxolo[5,6-c]-1,3-dioxolo[4,5-i]phenanthridinium) at 1-, 2-, and 5-µM doses was able to kill the cancerous tissue. DNA (deoxyribonucleic acid) ladder assay demonstrated that, compared to vehicle-treated control, sanguinarine treatment of squamous cell carcinoma resulted in an induction of programmed cell death as signaled by the nuclei in functioning cells. This process is characterized by cleavage of the DNA into fragments that give a so called laddering pattern then the solid phase of the cell liquefies.

The induction of apoptosis by sanguinarine was also especially clear when viewed with confocal microscopy (This microscope allows the observer to visualize objects in a single plane of focus, thereby creating a sharper image). This method identified the necrotic squamous cell carcinoma.

Sanguinaria Will Not Promote Necrosis To Healthy Skin Tissue
Sanguinarine treatment did not result in the formation of a DNA ladder or necrosis in normal human skin tissue. Necrosis did not occur even at the very high dose of 2 , 5, and 10 µM of sanguinarine. The results were viewed and verified with confocal microscopy.

The DNA cell cycle analysis showed that sanguinarine treatment did not significantly affect the distribution of cells among the different phases of the cell cycle in squamous cell carcinoma. (This is especially important because this proves definitely that sanguinarine will not affect the DNA of cells.) The researchers' work proves that sanguinarine is an effective natural anticancer chemical, and under normal circumstance sanguinarine will not promote damage to healthy tissue.

In fact, the safety of the product has been demonstrated with over 400 years of use by professionals and novices alike. Sanguinaria extract, sanguinarine, has been used in many over-the-counter products, including toothpaste (the anti-inflammatory properties in human's to reduce gingival inflammation and supragingival plaque.), mouthwash, cough and cold remedies, and homeopathic preparations for cancer removal.

The Search For Ancient Anticancer Chemicals Continues.
Agents that can eliminate the cancerous cells via a programmed cell death but do not affect the normal cells have a therapeutic advantage for the elimination of cancer cells.

Sanguinarine is a antiproliferative agent that has been developed as a anti-cancer agent as far back as 1600 A.D., by the peoples that inhabited North America which is currently the United States of America and Canada.

The Mechanism
Studies have shown that sanguinarine is a inhibitor of the activation of nuclear transcription factor NF-B, which has been implicated to play a key role in the regulation of cell growth, cell cycle regulation, and apoptosis. The anti-tumor properties of this alkaloid are constantly being reestablished.

At present, only a few agents are known to possess the potential for selective/preferential elimination of cancer cells without affecting the normal cells.

The University of Wisconsin studies provides more definitive evidence that sanguinarine at micromolar concentrations imparts a cell growth-inhibitory response in human squamous cell carcinoma and epidermoid carcinoma cells via an induction of apoptosis. In sharp contrast, normal human epidermal keratinocytes do not show any evidence of apoptosis, but undergo necrosis on treatment with higher concentrations of sanguinarine.
The University of Wisconsin research is the first study showing the complete cascade of events that lead to apoptotic cell death by sanguinarine. The University of Wisconsin researchers showed sanguinarine caused apoptosis of immortalized human Carcinoma tissue.

Chemistry Behind Sanguinarine's Apoptotic Cell Death
Normal keratinocytes is mediated via caspase activation triggered by modulations in Bcl-2 (B-cell lymphoma 2) family proteins-mediated cytochrome c release and the associated events. Bcl-2 is an anti-apoptotic gene. In fact, the link between apoptosis and cancer emerged when Bcl-2 , which is the gene that is linked to an immunoglobulin chromosome translocation pertaining to lymphoma, was found to inhibit cell death. This unexpected discovery gave birth to the concept that impaired apoptosis is a crucial step in the process of cancer development . In this study, The University of Wisconsin showed that sanguinarine treatment to the human Carcinoma tissue keratinocytes results in significant decrease in the levels of anti-apoptotic Bcl-2 protein and increases in the proapoptotic Bax protein. Thus shifting the Bax/Bcl-2 ratio in favor of apoptosis.

Studies have shown that Bcl-2 (i.e an enzyme that degrades DNA during apoptosis, inhibited by A molecule which represses or prevents another molecule from engaging in a reaction by the protein of 343 amino acids carrying a nuclear localization signal.) forms a heterodimer with Bax and might thereby neutralize its proapoptotic effects. In addition, Bcl-2 is also known to prevent the release of caspases. The University of Wisconsin studies have also shown the increase of protein levels of other proapoptotic members of Bcl-2 family, i.e., Bak and Bid, by sanguinarine treatment.

Furthermore, sanguinarine treatment of human Carcinoma tissue keratinocytes resulted in increase in the levels of cytochrome c (i.e. a protein which carries electrons that is central to the process of respiration in mitochondria (i.e. a small intracellular structurally discrete component of a cell which is responsible for energy production and the conversion within the cell of nutrients (such as protein molecules) into chemical energy in the form of ATP (adenosine triphosphate), by reacting the food with oxygen (O2) until the food has completely been degraded into carbon dioxide (CO2) and water (H2O.) and caspase.

These are important observations as it is known that the Bcl-2 family proteins (i.e. a proto-oncogene, activated by chromosome translocation in human B-cell lymphomas (hence bcl).. Encodes a plasma membrane protein. The gene product inhibits programmed cell death (apoptosis) and is homologous with the spiraling gene.) regulate the release of cytochrome c from the mitochondria into cytosol. Cytochrome c resides in the inter-membrane space of mitochondria, whereas its cofactors, Apaf-l and procaspase-9, are both cytosolic proteins. The over expression of Bcl-2 has been shown to block cytochrome c release in response to a variety of apoptotic stimuli. On the contrary, the pro-apoptotic members of Bcl-2 family proteins such as Bax, Bak, and Bid promote cytochrome c release from the mitochondria. The execution mechanism of apoptosis is mediated by caspases (cystein-yl aspartate-specific proteinases), which carry out the apoptotic program through a sequential activation cascade of initiator and executioner caspases. Apaf-1 induces activation of initiator caspase-9. Apaf-1 binds caspase-9 via the caspase recruitment domains at their NH2 termini, triggering the formation of a supramolecular complex. When activated, initiator caspase-9 triggers subsequent proteolytic activation of executioner caspase-3, caspase-7, and caspase-8. This whole process results in the cleavage of poly-adenosine diphosphate-ribose polymerase (i.e. an enzymes that catalyst the synthesis of nucleic acids on preexisting nucleic acid templates, assembling RNA from rib nucleotides or DNA from deoxyribonucleotides.) and subsequent DNA degradation and apoptotic death."

Contraindications
Contraindication from internally use: bloodroot should not be used if one is pregnancy, has high blood pressure, esophageal varicis, hiatus hernia, gastritis of peptic ulceration, and recent consumption of central nervous system stimulants. Use of emetics for more than three to four days can produce a serious medical condition if the assimilation of fluids is disrupted. This can lead to dehydration and severe electrolyte imbalances. Continual retching action from chronic emesis will strain the abdominal, gastric, and diaphragm muscles causing severe cramping and potential development of hernias.

Bloodroot used internally should not be administered to unconscious or deeply sedated individuals or in the event of convulsions, since bloodroot may cause aspiration of the gastric contents resulting in obstruction of the air passages.
Dan Raber

http://cancerres.aacrjournals.org/cgi/content/abstract/67/8/3888
Cancer Research 67, 3888-3897, April 15, 2007. doi: 10.1158/0008-5472.CAN-06-3764
© 2007 American Association for Cancer Research
Experimental Therapeutics, Molecular Targets, and Chemical Biology
Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells
Azhar R. Hussain1, Naif A. Al-Jomah1, Abdul K. Siraj1, Pulicat Manogaran2, Khalid Al-Hussein2, Jehad Abubaker1, Leonidas C. Platanias3, Khawla S. Al-Kuraya1 and Shahab Uddin1

1 Human Cancer Genomic Research, King Fahad National Center for Children's Cancer and Research, 2 Biological and Medical Research, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia and 3 Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, Northwestern University Medical School, Chicago, Illinois

Requests for reprints: Shahab Uddin, King Faisal Specialist Hospital and Research Cancer, King Fahad National Center for Children's Cancer and Research, MBC#98-16, P.O. Box 3354, Riyadh 11211, Saudi Arabia. Phone: 966-1-205-5169; Fax: 966-1-205-5170; E-mail: Shahab@kfshrc.edu.sa.

Primary effusion lymphoma (PEL) is an incurable, aggressive B-cell malignancy that develops rapid resistance to conventional chemotherapy. In efforts to identify novel approaches to block proliferation of PEL cells, we found that sanguinarine, a natural compound isolated from the root plant Sanguinaria canadendid, inhibits cell proliferation and induces apoptosis in a dose-dependent manner in several PEL cell lines. Our data show that sanguinarine treatment of PEL cells results in up-regulation of death receptor 5 (DR5) expression via generation of reactive oxygen species (ROS) and causes activation of caspase-8 and truncation of Bid (tBid). Subsequently, tBid translocates to the mitochondria causing conformational changes in Bax, leading to loss of mitochondrial membrane potential and release of cytochrome c to the cytosol. Sanguinarine-induced release of cytochrome c results in activation of caspase-9 and caspase-3 and poly(ADP-ribose) polymerase (PARP) cleavage, leading to induction of caspase-dependent apoptosis. In addition, we show that pretreatment of PEL cells with carbobenzoxy-Val-Ala-Asp-fluoromethylketone, a universal inhibitor of caspases, abrogates caspase and PARP activation and prevents cell death induced by sanguinarine. Moreover, treatment of PEL cells with sanguinarine down-regulates expression of inhibitor of apoptosis proteins (IAP). Finally, N-acetylcysteine, an inhibitor of ROS, inhibits sanguinarine-induced generation of ROS, up-regulation of DR5, Bax conformational changes, activation of caspase-3, and down-regulation of IAPs. Taken together, our findings suggest that sanguinarine is a potent inducer of apoptosis of PEL cells via up-regulation of DR5 and raise the possibility that this agent may be of value in the development of novel therapeutic approaches for the treatment of PEL. [Cancer Res 2007;67(8):3888–97]

[

Cancer Research 66, 3726-3736, April 1, 2006]
© 2006 American Association for Cancer Research
Cell, Tumor, and Stem Cell Biology
Cyclooxygenase 2 Rescues LNCaP Prostate Cancer Cells from Sanguinarine-Induced
Apoptosis by a Mechanism Involving Inhibition of Nitric Oxide Synthase Activity
Jacob Huh1, Andrejs Liepins1,{dagger}, Jacek Zielonka2, Christopher Andrekopoulos2, Balaraman Kalyanaraman2 and Andrey Sorokin1

Departments of 1 Medicine and 2 Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin

Requests for reprints: Andrey Sorokin, Department of Medicine, Medical College of Wisconsin, CVRC, 8701 Watertown Plank Road, Milwaukee, WI 53266. Phone: 414-456-4438; Fax: 414-456-6515; E-mail: sorokin@mcw.edu.

Expression of cyclooxygenase-2 (Cox-2), an inducible enzyme responsible for the production of prostaglandins from arachidonic acid, is elevated in human prostate tumor samples. The aim of this study was to investigate whether expression of Cox-2 is effective against prostate cancer cell apoptosis triggered by sanguinarine, the quaternary benzophenanthridine alkaloid with antineoplastic properties. Sanguinarine effectively induced apoptosis in LNCaP human prostate cancer epithelial cells as assessed by caspase-3 activation assay, Annexin V staining assay, or by visual analysis for the apoptotic morphology changes. Sanguinarine-mediated apoptosis was associated with the increase of nitric oxide (NO) formation in prostate cancer cells as assessed by measurements of nitrites with Sievers nitric oxide analyzer as well as flow cytometry analysis using NO fluorescent sensor. Activation of NO synthase (NOS) activity was crucial for sanguinarine-induced cell death because NOS inhibitor L-NMMA efficiently protected cells from apoptosis. Adenovirus-mediated transfer of Cox-2 into LNCaP cells inhibited sanguinarine-induced apoptosis and prevented an increase in NO production. Surprisingly, NO donors failed to induce apoptosis in LNCaP cells, suggesting that constitutive NO generation is not sufficient for triggering apoptosis in these cells. Besides NO generation, NOS is also capable of producing superoxide radicals. Sanguinarine-induced production of superoxide radicals, and the addition of MnTBAP, a scavenger of superoxide radicals, efficiently inhibited sanguinarine-mediated apoptosis. These results suggest that Cox-2 expression rescues prostate cancer cells from sanguinarine-induced apoptosis by a mechanism involving inhibition of NOS activity, and that coadministration of Cox-2 inhibitors with sanguinarine may be developed as a strategy for the management of prostate cancer. (Cancer Res 2006; 66(7): 3726-36)

Originally published In Press as doi:10.1074/jbc.M501467200 on March 7, 2005
J. Biol. Chem., Vol. 280, Issue 19, 19078-19086, May 13, 2005
The Benzo[c]phenanthridine Alkaloid, Sanguinarine, Is a Selective, Cell-active Inhibitor of Mitogen-activated Protein Kinase Phosphatase-1*
Andreas Vogt{ddagger}, Aletheia Tamewitz, John Skoko, Rachel P. Sikorski, Kenneth A. Giuliano§, and John S. Lazo
From the Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

Mitogen-activated protein kinase phosphatase-1 (MKP-1) is a dual specificity phosphatase that is overexpressed in many human tumors and can protect cells from apoptosis caused by DNA-damaging agents or cellular stress. Small molecule inhibitors of MKP-1 have not been reported, in part because of the lack of structural guidance for inhibitor design and definitive assays for MKP-1 inhibition in intact cells. Herein we have exploited a high content chemical complementation assay to analyze a diverse collection of pure natural products for cellular MKP-1 inhibition. Using two-dimensional Kolmogorov-Smirnov statistics, we identified sanguinarine, a plant alkaloid with known antibiotic and antitumor activity but no primary cellular target, as a potent and selective inhibitor of MKP-1. Sanguinarine inhibited cellular MKP-1 with an IC50 of 10 µM and showed selectivity for MKP-1 over MKP-3. Sanguinarine also inhibited MKP-1 and the MKP-1 like phosphatase, MKP-L, in vitro with IC50 values of 17.3 and 12.5 µM, respectively, and showed 5–10-fold selectivity for MKP-3 and MKP-1 over VH-1-related phosphatase, Cdc25B2, or protein-tyrosine phosphatase 1B. In a human tumor cell line with high MKP-1 levels, sanguinarine caused enhanced ERK and JNK/SAPK phosphorylation. A close congener of sanguinarine, chelerythrine, also inhibited MKP-1 in vitro and in whole cells, and activated ERK and JNK/SAPK. In contrast, sanguinarine analogs lacking the benzophenanthridine scaffold did not inhibit MKP-1 in vitro or in cells nor did they cause ERK or JNK/SAPK phosphorylation. These data illustrate the utility of a chemical complementation assay linked with multiparameter high content cellular screening.

Received for publication, February 8, 2005

* This work was supported by National Institutes of Health Grants CA78039 and CA52995 and the Fiske Drug Discovery Fund. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Current address: Cellumen, Inc., 100 Technology Dr., Pittsburgh, PA 15219.

{ddagger} To whom correspondence may be addressed: Dept. of Pharmacology, The Hillman Cancer Center G.27a, University of Pittsburgh, Pittsburgh, PA 15213. Tel.: 412-623-1216; Fax: 412-623-1212; E-mail: avogt@pitt.edu.
To whom correspondence may be addressed: Dept. of Pharmacology, Biomedical Science Tower E-1340, University of Pittsburgh, Pittsburgh, PA 15261. Tel.: 412-648-9319; Fax: 412-648-2229; E-mail: lazo@pitt.edu.

http://www.cancerx.org/pancreatic_cancer_cure.html
A Cure of Pancreatic Cancer?
Diagnosed pancreatic cancer will touch the lives of an estimated 33,730 persons in 2006; in the same year 32,300 deaths will occur, 3 based on current pancreatic cancer health trends… to put this into context 2.26 persons per 100 should survive after 5 years using allopathic treatments. This statistic demands that allopathic treatments be re-examined, when the best they offer is a 98 percent death rate.

Technically Speaking
When using Sanguinarine the mitochondria is the target for the destruction of cancer. 4 Studies showing the modulation of mitochondrial events and the process of apoptosis by bloodroot are useful in phytochemicals prevention as well as phytochemicals therapy of cancer and possibly other
hyper proliferative disorders .Pancreatic cancer AsPC-1 and BxPC-3 cells studied at the University of Wisconsin, demonstrated that sanguinarine (bloodroot) treatment to AsPC-1and BxPC-3 cells resulted in a dose dependent inhibition of cell growth (up to 62% in AsPC-1 and 47.3% in BxPC-3 cells at 10 µM)inhibition of cell viability up to 97.3% in

AsPC-1and 95.6% in BxPC-3 cells at 10 µM arrest of cells in G0-G1 phase of the cell cycle up to 64.7% in AsPC-1 and 74.1% in BxPC-3 cells at 10 µM induction of apoptosis 97.9% in AsPC-1 and 96.5% in BxPC-3 cells at 10 µM.

Please note the bloodroot extract was present for 24 hours before testing. 4

1.http://www.cancer.org/.......
2. 2006 American cancer Society, Inc No. 500606
3. http://www.cancer.gov/.......
4. Experimental and Molecular Therapeutics Novel Agents 1 Abstract #629

Prostate Cancer e-mail: FlowWOOLF@swbell.net for products

* Prostate Cancer Prevalence: Prostate cancer is the second most common cancer in the U.S.; it is the second leading cause of cancer-related death to American men. In 2002, an approximate 189,000 men were diagnosed with, and 30,200 men died from this disease. African American men are more likely than other racial and ethnic populations both to develop and to die from PC. (footnote 9). Scientific evidence suggests that about one-third of PC could have been averted with proper nutrition, (footnote 2) e.g., foods rich in antioxidants and immune stimulating substances such as co-enzyme Q10. Historical and emerging research also shows that supplementing with herbs that have been proven to change the structure/function of PC cells can be the key to managing this disease. (footnote 3-5, 11)

* Studies: In a study, Sanguinarine was tested for the management of PC. Mice were implanted with human PC tumors. The study demonstrated the preventive and post therapeutic effects of sanguinarine against PC. (footnote 10) A study from the Czech Republic analyzed sanguinarine and proved that sanguinarine is an excellent herb which can deliver therapeutic results to men burdened with PC. Sanguinarine enhances the immune system by means of utterly destroying cancer cells. (footnote 11)

* The Science of Curing Prostate Cancer: Sanguinarine destroys cancer cells. (footnote 3-5, 7-11) Sanguinarine has been shown to cause cell cycle blockade and apoptosis of human prostate cancer cells irrespective of their androgen status. This is an important finding, because prostate cancer is known to undergo a transition from an early “androgen-sensitive” form of cancer to a late (metastatic) “androgen-insensitive” form of cancer. At diagnosis, most prostate cancer represents a mixture of androgen-sensitive and androgen-insensitive cells. Therefore, the key to the control of prostate cancer seems to lie in the elimination of both types of prostate cancer cells (without affecting the normal cells) via mechanism-based preventive/therapeutic approaches. (footnotes 3-5) Overall, the results of studies indicate that sanguinarine is a useful agent in the destruction of PC.

* Conclusion: Persons with cancer should take a multi-function approach to repairing their body.

First, by using Pancreatic Enzyme Therapy, TumorXTM Formulas (103X, 303X, & 403X), to destroy cancer cells.6

Second, by using Formulas ApoptosisTM Full Strength, & Easy Digest, which contain the life saving phyto-nutrient sanguinarine which has been proven to destroy cancer cells.(footnotes 3-5)

Third, by the addition of Formula Pure Energy ATPTM (footnote 7) which allows the body to convert the poisonous waste of cancer into energy.

And fourth, by using BioEnerGetics Q-10,TM 8 an antioxidant scavenger which stimulates the immune system.

Resources

1. http://www.cdc.gov/cancer/prostate/decisionguide/index.htm

2. 2006 American cancer Society, Inc No. 500606

3. Mol Cancer Ther. 2004;3:933-940

4. Proc Amer Assoc Cancer Res, Volume 46, 2005

5. Cancer Res 2006; 66: (7). April 1, 2006

6. Pancreas. 28(4):401-412, May 2004.

7. Journal of the National Cancer Institute, Vol. 92, No. 4, February 16, 2000

8. National Cancer Institute Questions and Answers About Coenzyme Q10

9. Prostate Cancer Progress Report 2004 National Cancer Institute of Health

10. Sanguinarine: A Novel Agent Against Prostate Cancer. - Annual rept. 16 Jan 2004-15 Jan 2005.

11. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006, 150(1) 5–12.

12. DOD award number W81XWH-04-100220 TITLE: Sanguinarine: A Novel Agent Against Prostate Cancer

Bloodroot Information:
http://www.med.unc.edu/phyrehab/ncmedicinalherbs/Bloodroot/Bloodroot-cg.pdfProgram on Integrative Medicine, Department of Physical Medicine and Rehabilitation,
School of Medicine, University of North Carolina, Chapel Hill, NC 27599-7200. http://pim.med.unc.edu/
Prepared by Faurot KR, Kroll DJ, Curtis P, Greenfield JT, Siegel SY, Gaylord SA, Mann JD
For the NC Consortium on Natural Medicines-funded by a grant from the GoldenLEAF Foundation

A monograph using the following references:

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17. Mills S, Bone K. Principles and Practice of Phytotherapy. Philadelphia: Churchill Livingstone, 2000.
18. Simard F, Landry RG. Mouthrinses as an antibacterial adjunct in periodontal treatment. J Can Dent Assoc 60 (10): 906-7, 910-1, 1994.
19. Sturdivant L, Blakeley T. The Bootstrap Guide to Medicinal Herbs in the Garden, Field, & Marketplace. Friday Harbor, WA: San Juan Naturals, 1999.
20. Tenenbaum H, Dahan M, Soell M. Effectiveness of a sanguinarine regimen after scaling and root planing. J Periodontol 70 (3): 307-11, 1999.
21. Thornton L. Wildcrafters Alert! Aromatic News: Herbal Rose Report, pp. 4A, 1999.
22. Tierra M. The Way of Herbs. New York: Pocket Books, 1998.

OUR PERSONAL EXPERIENCES AND GENERAL HEALTH INFORMATION

A family member has used the salve four times starting in 1986. He was an alcoholic (now in recovery, 2007) diagnosed with cirrhosis of the liver and wanted to try to salve for his diseased liver--a use we had never heard of. The site advised for use when the diseased area is internal and not near the skin is on the groin, just below the belly and above the center of the leg. The first two times were easy—little pain or discomfort except from bandages and tape and skin that became very sensitive. He could feel the salve pulling from several areas in his body, from the liver area, from his tobacco smoke-damaged lungs, from areas where he had been injured, and especially from several areas of former severe infection from vaccine-caused strept A flesh-eating bacterial infection supposedly “cured” with antibiotics, which never healed properly. However, on the 3rd treatment, he became severely ill, couldn’t keep down food or even tap water (we had to get purified water), and he passed black, tarry matter from the bowels.

We drove 200 miles to a well-known naturopathic physician who determined that it was just a detoxing reaction. She gave him liver protomorphogens (glandular) medicine to support liver function and detoxification. She was right. The illness passed in a few days, and he felt remarkably better afterwards. That was over three years ago and he is just now feeling the need to do the treatment again—probably due to his continued use of harmful substances like tobacco and alcohol.

He does more than the salve treatment to support the healing process. He had “constitutional food intolerances” diagnosed (related to blood type intolerances) by the same naturopathic physician, Dr. Leticia Watrous in Spokane, Washington. (Dr. Joel Wallach, author of best-selling audio tape “ DEAD DOCTORS DON’T LIE” describes how to self-diagnose food allergies in his books-- food allergy testing), and he follows the prescribed diet religiously, as that removes a tremendous source of toxicity and stress to the system. He also follows the general blood type diet suggestions in Dr. D’Adamo’s book “4 diets for 4 blood types.” This has made an extraordinary difference in the family's overall health. Added to that, their diet is primarily raw, except for meat and potatoes, and low carb, which is necessary for improved health in most individuals. At least low in refined carbs, which are dangerous in general.

They also take numerous supplements. Vitamins are present in raw foods because they are created in plant life from sunlight and water. However, the commercial soils of the earth have been long depleted of most of the minerals we need for optimum health. One of the most important is calcium, which is made bio-unavailable when heated, such as in the process of pasteurization, which also makes it more acidic, so milk actually strips more calcium from the body’s resources than it provides. One must supplement minerals or die—it’s that simple, unless you happen to live in one of those small pockets of areas in the world far from civilization where the waters are glacier-fed and food organically grown and people live and work to age 120, as our cells are programmed to do when provided with all the building materials for health.

A short list of most commonly used supplements is available online—they are laboratory-tested and sold for basically non-profit wholesale prices at: ourhealthcoop.com.

Calcium, unless from plant sources like carrots or dark green leafy vegetables, is hard to metabolize and in supplement form may even contribute to deadly arterial plague. A doctor who wrote a book on curing arthritis names calcium supplementation as a necessary part of the treatment, but says it needs certain things to make it bio-available. First—sunlight on the oil-producing areas of the skin for natural vitamin D. No synthetic can possibly replace what sunlight provides. However, he suggests taking a tablespoon of cod liver oil (flavored Norwegian Cod Liver Oil in a little juice) at night. In the morning, a tablespoon of raw, cold-pressed peanut oil (from health food stores). During the day, divide your calcium into 3 or 4 doses, and take it with an acid drink (like citrus fruit juice) to which is added vitamin C crystals or a tablespoonful of apple cider vinegar. (We use lemon juice sweetened with Stevia vegetable sweetener and apple cider vinegar with the "mother", or go right to a honey and vinegar drink.) It is also suggested taking HCL (hydrochloric Acid—stomach/digestive acid) with it, along with pancreatic enzymes and digestive enzymes. But for all of the minerals combined, we use SeaSilver, or something similar like the new "Perfect Food" from Garden of Life, etc. Mainly, get some kind of organically-grown, food-based enzyme rich “SUPER FOOD.” Virtually every health food store in the country carries something referred to as such. They’ll know what you mean immediately.

Some other excellent products are “Km,” a potassium-rich drink made with roots, berries, and the like, created and manufactured in Canada. It's available over the internet. It’s one of the few supplements my family has ever used and seen a visible difference. Thick pads of skin on the bottom of one person's feet softened into normal skin, hair got shinier, and nobody had dental carries for several years after using it. I figure if we could actually see changes, that the unseen changes were there, also. You can order this product from distributors online.

Minerals occur in ratios to each other. The “big 4” are calcium, magnesium, potassium, and sodium. Most people are deficient in some or all of these, along with all the missing trace minerals no longer in the soils that produce our foods. Some people need more calcium in relation to magnesium, or the other way around. The best way to find out for certain what you need or have too much of is to locate some kind of health practitioner (many chiropractors as well as alternative doctors are doing amazing things in the areas of diagnostic work) who can send away a hair sample for mineral analysis. The best-known laboratory in the world of this kind, and its famous doctor/researcher is A.R.L. with Dr. Paul Eck, a pioneer in the field whom most other such diagnostic laboratories and their researchers base their work on.

We also take digestive enzymes after eating (HCL before), and PANCREATIC ENZYMES—a deficiency which many healers consider plays a pivotal role in the development of cancer and other diseases, especially colon cancer.

Avoid HYDROGENATED OILS at all costs. JUST SAY NO!!!!!! We are a world desperately insufficient in our intake of omega 3 & 6 oils, such as from fish oil and flax seed oil, and these manmade oil forms “trick” the oil-starved body into thinking its the “real” thing. They absorb these atrocities into the cells, which glom them up and destroy the cell’s electrical charge which attracts oxygen into the cell. No oxygen=cell death or cell mutation into cancer. Read the work of Dr. Johanna Budwig for the research and the solutions. Any internet search engine will take you to this shocking, life-saving information. We use pure, natural extra-virgin olive oil for cooking and eating (your heart and vascular system love this), and take fish and flaxseed oil for medicine.

The human body must have all 12 cell (tissue) salts which have been long made in homeopathic preparations: Dr. Schussler’s Tissue Salts, found in many health food stores. They come individually, or all 12 in 1, called “BIOPLASMA,” which are healing in themselves. There exists a definitive “Bible” on the subject called “Tissue Salts” or something like that, and it relates all of the symptoms and diseases that can arise from an imbalance or deficiency of any one of these necessary substances.

We use natural glandulars as the need has been diagnosed by healing professionals. My husband has needed liver support and so used the liver “protomorphogens”—the part of the glandular cells that heal them and make them work, and when my adrenals crashed, taking down my thyroid with them and affecting virtually every function and organ in my body, instead of going on synthetic thyroid hormone and suppressing or destroying my own remaining thyroid function, or cortisone, which would both shorten and affect the quality of my life, I used the glandulars, cleansing protocols, and a massive healing effort which eventually restored my health.

Cancer usually involves poor liver function. One needs to go through cleansing and detoxing procedures, particularly designed to restore liver function, since it is the primary agent of filtering and detoxification in your body. The best information and website I’ve found on essential cleansing/detoxing programs is this: http://www.healthfree.com/health/

For additional cleansing and detoxing, we use ESSIAC tea, now available in most health food stores made up or in loose powdered tea form that you make up yourself. Follow the directions carefully, there are specific steps to take in making the tea, which requires an all-day process. Several other famous teas reportedly have similar properties, such as Pau D’Arco tea. Also, mushroom tea (Kombucha tea, as described in the book “Kombucha” by Gunther W. Frank) , which you brew at home with a Kombucha mushroom or starter kit, has a wealth of nutrients and reported healing properties. This is amazing stuff that tastes like a cross between sparkling cider, vinegar, and cheap wine, and gives most people an energy lift immediately. Enervation is a serious condition of low nerve energy that needs to be remedied for any condition of health to be restored.

One simple, natural treatment that has reportedly been used alone successfully as a cancer treatment is the carrot/celery/beet juice mix--raw, fresh vegetables, organically grown if possible. Add selenium, zinc and vitamin E to this mix and you have a healing “elixir.” Whether it’s the calcium, beta-carotene, fresh enzymes, or whatever, these home-juiced vegetables come close to being “miracle” foods. I cheat—the health food store sells powdered carrots and beets which I occasionally substitute, and I admit to going VERY lightly on the beets (they taste gross), but this is the kind of food that makes the best medicine.

Parasites: There exists a large body of documented evidence that parasites play a part in some if not most cancers. How about when they find at the dead center of a tumor a bolus of parasites, dead or otherwise, or find that some excrete toxic substances that trigger malignancy in benign tumors. There are many websites devoted to the subject of parasite treatment, particularly those involving the research of scientist DR HULDA CLARK, who is spite of being infamous and controversial, has impeccable research skills. Most parasite cleanses include the use of green walnut hull, wormwood and cloves—the non-irradiated type you purchase elsewhere than your favorite local grocery store. Also, the remedies of the late HANNA KROGER, whose work and medicines continue to be produced at: 7075 Valmont Rd., Boulder CO 80301.

Salve treatment guidence: http://blacksalveinfo.com Rick from the black salve site appears willing to advise anyone using salves from any source, although he has links to salve products. One of the best instruction pages I've seen along with Dan Raber's site, is on the web page at Rick's site: http://blacksalveinfo.com/instrbs.htm This is geared more for skin cancers, but the treatment is the same, and he must not realize that deeper cancers can also be treated, as has been our experience.

Medical salve use: In the Philippines, medical doctors administer salve treatments as part of their own "orthodox" medical arsenal. Bloodroot salve treatments have been in use since 2002: "Jade Del Mundo Md. From the Department of Health invites you to the Philippines for your healing." (info from the cancerx.org site)

ESCAROTIC CANCER SALVES (bloodroot variety)

(Disclaimer: This material is intended for information only and not given as medical advice, which should come from a licensed health care provider.)

The following formulas for light and dark salves used alternately in an old herbal cancer treatment was submitted to the Yakima Herald-Republic around 1980 by a Yakima Valley chiropractor that promptly lost his license to practice his profession legally because of it.

He reportedly owns a 100+ year old book with this remedy included in it, said to have originated with Canadian Indians, but of which many varieties go way back in time, particularly since the herb bloodroot has been known for it’s tumor-fighting properties since ancient times.

A family friend cut it out and kept it, but we came across information about it from friend of the family who heard about this cancer research project and reported that she had been diagnosed with metasticized breast cancer and sent home from the hospital with instructions to “put her affairs in order” as she was scheduled (by them) to die.

Someone who knew her insisted on giving her the salve treatment. Having nothing to lose, she submitted to the procedure. Later, the same doctors who pronounced the death sentence insisted that it must have been “a misdiagnosis.” No sign of the cancer remained, and she has been cancer-free for many years now.

The above case was mentioned to the former owner of a local health food store. She knew about the salves and someone else who had been using them. The person who used the salve on herself--Bernice Dodd--is the one who gave us the formula in 1996, along with a small container of “black salve”—the first part of this 2-salve (light and black salve) treatment. We made the other one ourselves from the instructions below.

The lady with the breast cancer only used the salve one time but checks every year to see if she gets a positive reaction from it (if left on the skin for a 24 hour period, the black salve will either do nothing or cause a skin reaction, indicating the presence of diseased tissue.)

The family member who has used the treatment several times probably has some cancer or pre-cancerous condition on-going and the treatment keeps it cleaned up. He was also diagnosed with cirrhosis of the liver over 22 years ago, which also seems not to have progressed. In fact, there have been no symptoms of it for many years.

Since this formula was published in our county newspaper, a comprehensive book has been written on the subject of “cancer salves” of which there are many more than the two described here. The author has also created a massive website, so much of this information is available and the book and links to actual prepared formulas which can be purchased are all available online at: